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Mech Ageing Dev. 2011 Apr;132(4):171-9. doi: 10.1016/j.mad.2011.03.002. Epub 2011 Mar 29.
Differential cysteine depletion in respiratory chain complexes enables the distinction of longevity from aerobicity.
Schindeldecker M1, Stark M, Behl C, Moosmann B.
Author information
Evolutionary Pathobiochemistry Group, University Medical Center of the Johannes Gutenberg University, Duesbergweg 6, Mainz, Germany.
Abstract
Mitochondrially encoded proteins in long-lived animals exhibit a characteristic anomaly on the amino acid usage level: they abstain from the use of cysteine in a lifespan-dependent fashion. Here, we have further investigated this phenomenon by analyzing respiratory chain complex subunits individually. We find that complex I cysteine depletion is the almost exclusive carrier of the cysteine-lifespan correlation, whereas complex IV cysteine depletion is uniform in all aerobic animals, unrelated to longevity, but even more pronounced than complex I cysteine depletion in the longest-lived species. In nuclear encoded subunits of the respiratory chain, we find lifespan-independent cysteine depletion ranging from moderate in complex I to severe in complex V. However, a separate analysis of the transmembrane domains of these proteins unmasks an intramembrane pattern of cysteine usage that significantly correlates with longevity. Hence, cysteine usage in the respiratory chain seems to be governed by two independent molecular mechanisms acting on the protein level: a global trend of cysteine avoidance in all proteins, and a specific effect on transmembrane domain cysteines that reflects longevity. To account for these characteristic patterns, a thiyl radical-based molecular mechanism of intramembrane hydrophobic cross-linking is discussed.
Begin_電腦自動翻譯的內容_Y2018m11d04h16m17Rn7055_
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抽象
在長壽命動物中粒線體編碼的蛋白質在氨基酸使用水平上表現出特徵異常：它們以生命依賴的方式避免使用半胱胺酸。在這裡，我們通過分別分析呼吸鏈複雜亞基進一步研究了這一現象。我們發現複雜的I半胱胺酸消耗是半胱胺酸 - 壽命相關性的幾乎唯一的載體，而復雜的IV半胱胺酸消耗在所有需氧動物中是均勻的，與長壽無關，但在最長壽命的物種中比複雜的I半胱胺酸消耗更加明顯。。在呼吸鏈的核編碼亞基中，我們發現不依賴於生命週期的半胱胺酸消耗，從復合物I中的中度到復雜的V中的嚴重。但是，對這些蛋白質的跨膜結構域的單獨分析揭示了半胱胺酸使用的膜內模式，其顯著相關長壽。因此，呼吸鏈中的半胱胺酸使用似乎受兩種作用於蛋白質水平的獨立分子機制控制：所有蛋白質中半胱胺酸避免的全球趨勢，以及反映壽命的跨膜結構域半胱胺酸的特定作用。為了解釋這些特徵模式，討論了基於硫基的膜內疏水交聯的分子機制。
End_電腦自動翻譯的內容_Y2018m11d04h16m17Rn7055_
PMID: 21453719 DOI: 10.1016/j.mad.2011.03.002
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Differential cysteine depletion in respiratory chain complexes enables the distinction of longevity from aerobicity. [2011]; Germany;_WJD_2018-1104_V001R01_IR94_
Source (資訊來源):
Info cited on 2018-11-04-WD7 (資訊引用於 中華民國107年11月4日) by 湯偉晉 (WeiJin Tang)
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