ChatGPT 負責翻譯：Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection PY1995 IR94 IR95 台中榮總研發部，穀胱甘肽，血漿， 台灣的男人和女人
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1995
Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection
Affiliation
Department of Medical Research. Taichung Veterans General Hospital Taiwan
PMID: 8749248
Abstract
A convenient method for the determination of reduced glutathione (GSH) and oxidized glutathione (GSSG) in human plasma by high-performance liquid chromatography with fluorescence detection is reported. This assay involves direct addition of human plasma to methanolic monobromobimane, for simultaneous protein precipitation and thiol derivatization. The assay was validated by addition of authentic GSH and GSSG to plasma samples. Plasma glutathione levels in Chinese male and female volunteers were found to decrease with increasing age (age groups 20-30, 30-40, 40-50, 50-60, and > 60; mean +/- S.E.M. 0.95 +/- 0.03, 0.77 +/- 0.02, 0.67 +/- 0.03, 0.51 +/- 0.02, 0.48 +/- 0.02 microM for male volunteers and 1.11 +/- 0.06, 0.76 +/- 0.03, 0.61 +/- 0.03, 0.53 +/- 0.04 and 0.43 +/- 0.04 microM for female volunteers). GSSG levels, in both males and females, did not show a correlation with age. There were no significant differences in GSH or GSSG levels among male and female volunteers of the same age group. These results suggest that elderly persons might be more susceptible to oxidative injury due to decreased plasma glutathione levels.
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Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection
(透過高效液相層析與螢光檢測法測定老化對人類血漿穀胱甘肽濃度的影響)
C S Yang
(C S Yang)
Affiliation
(隸屬單位)
Department of Medical Research, Taichung Veterans General Hospital, Taiwan
(台中榮民總醫院醫學研究部，台灣)
PMID: 8749248
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Abstract
(摘要)
A convenient method for the determination of reduced glutathione (GSH) and oxidized glutathione (GSSG) in human plasma by high-performance liquid chromatography with fluorescence detection is reported.
(本研究報導了一種利用高效液相層析（HPLC）搭配螢光檢測法測定人類血漿中還原型穀胱甘肽（GSH）與氧化型穀胱甘肽（GSSG）的便捷方法。)
This assay involves direct addition of human plasma to methanolic monobromobimane, for simultaneous protein precipitation and thiol derivatization.
(該檢測方法透過將人類血漿直接加入甲醇單溴雙美（monobromobimane），以同時進行蛋白質沉澱與巰基（thiol）衍生化。)
The assay was validated by addition of authentic GSH and GSSG to plasma samples.
(此方法的有效性已透過在血漿樣本中加入標準 GSH 和 GSSG 進行驗證。)
Plasma glutathione levels in Chinese male and female volunteers were found to decrease with increasing age.
(在中國籍男性與女性受試者中，血漿穀胱甘肽濃度隨年齡增加而下降。)
(Age groups 20-30, 30-40, 40-50, 50-60, and > 60; mean +/- S.E.M.: 0.95 +/- 0.03, 0.77 +/- 0.02, 0.67 +/- 0.03, 0.51 +/- 0.02, 0.48 +/- 0.02 μM for male volunteers and 1.11 +/- 0.06, 0.76 +/- 0.03, 0.61 +/- 0.03, 0.53 +/- 0.04 and 0.43 +/- 0.04 μM for female volunteers).
(各年齡組（20-30、30-40、40-50、50-60 及 >60 歲）的血漿穀胱甘肽濃度（平均值 ± S.E.M.）為：
- 男性受試者：0.95 ± 0.03、0.77 ± 0.02、0.67 ± 0.03、0.51 ± 0.02、0.48 ± 0.02 μM
- 女性受試者：1.11 ± 0.06、0.76 ± 0.03、0.61 ± 0.03、0.53 ± 0.04、0.43 ± 0.04 μM。)
GSSG levels, in both males and females, did not show a correlation with age.
(在男性與女性受試者中，GSSG 濃度與年齡之間未呈現相關性。)
There were no significant differences in GSH or GSSG levels among male and female volunteers of the same age group.
(相同年齡組別內，男性與女性受試者的 GSH 和 GSSG 濃度無顯著差異。)
These results suggest that elderly persons might be more susceptible to oxidative injury due to decreased plasma glutathione levels.
(這些結果表明，老年人可能因血漿穀胱甘肽濃度下降而更容易受到氧化損傷。)
(中文由 ChatGPT 負責翻譯)
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